pcb 3063 lab report 1

Visible areas of crossing over (chiasmata) and homologous chromosomes pull apart, Diakinesis substage of meiotic prophase 1, Separate! 0 Prerequisite: AGR 3303 or PCB 3063 . 3 0 obj By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. Course Hero is not sponsored or endorsed by any college or university. 0. - c()g7{5EHhHR3G}Ug:bi X(O]m:]by6=hqk:,}9 PCB3063L Lab Report 1.docx 4 pages PCB3063L+Lab+Report+2+Draft+and+Full+Report+Instructions (2).pdf 2 pages PCB3063L Online Exp Design activity Instruction Sheet.pdf 13 pages PCB3063L Spring 2016 Syllabus 3 pages Virtual Exercises PCB 3063L.docx 6 pages Population+Genetics+PCB+3063L copy.docx 2 pages Red+Cone+Opsin+Part+II+PCB+3063L.docx 2 pages In our lab notebooks, we wrote hypothesiss as to Two copies of the same form of gene (Ex. (20+), Ch 1, Section 1.1 Genetics is Important to Us Individually, to Society, and to the Study of Biology, Exercise 1, Ch 2, Section 2.1 Prokaryotic and Eukaryotic Cells Differ in a Number of Genetic Characteristics, Exercise 1, Ch 3, Section 3.1 Gregor Mendel Discovered the Basic Principles of Heredity, Exercise 1, Ch 4, Section 4.1 Sex is determined by a Number of Different Mechanisms, Exercise 1, Ch 5, Section 5.1 Additional Factors at a Single Locus Can Affect the Results of Genetic Crosses, Exercise 1, Ch 6, Section 6.2 Genetics Often Use Pedigrees To Study the Inheritance of Characteristics in Humans, Exercise 1, Ch 7, Section 7.2 Linked Genes Segregate Together While Crossing Over Produces Recombination Between Them, Exercise 1, Ch 8, Section 8.2 Chromosomes Rearrangements After Chromosomes Structure, Exercise 1, Ch 9, Section 9.2 Genetic Analysis of Bacteria Requires Special Methods, Exercise 1, PCB 3063L Heterozygous X-linked loci, Chromosomal genotype. Drosophila melanogaster: Determination of an Unknown Genotype Using Mendelian \vCnIGx%Jf ]{J,.}]dDT9"t6sp8i!6@w8bXj;CjyfawdC by]bQT|mBZ |al5=:B^ihGjsWR4 LT$[^^%2y;gY=;]~'KZhU\UpbsbS W R*deO?mI?pd:#!ll^ k8{;b^-oW];).}v.oV :&1F>4ta72DC&ds@r=8H)\chYCz{ {KgwQ?[fio/u|u;cGnFXcSUsXln007_}Fx4Bn7M?Q47gAzfQW`,fCq,}+{(u"xjmhI[f->`(fg7F)G bV,GLMw5m#2}tcF~(2 The flies were then anesthetized for transfer and scoring, utilizing Fly-nap, a commercial fly anesthetic (Garey et al. Why? Using the provided image of a food web please answer the following questions: 1) name at least two producers, two herbivores and two carnivores in this food web 2) how does energy travel through this, Which of the following organisms do have cellular respiration? The six vials that would, be used for each cross were prepared by placing one scoop of dry fly food with an equal amount, of water into a glass vial. first table reveals 50% of the offspring having dominant red eyes (wildtype phenotype) and the (672 Documents), PCB 3023L - usf With these genotypes, we had to construct an and Pigment Biosynthesis Pathways in Drosophila melanogaster. Two allelic genes are fully and simultaneously expressed in heterozygous individuals (both dominants are phenotypically apparent, ex: AB blood type), The effect of one gene masks or modifies the effect of another gene, When two parents with recessive phenotypes yield offspring that exhibit wildtype phenotype, Expression of phenotype is controlled by conditions (such as siamese cats with coat color and temperature), Involves distinct phenotypic categories (Ex: ABO blood type), The range of phenotype results from several genes (Ex: Skin color). team. With these tables, they reveal our predicted offspring PCB3063L Online Lab Report 2 Draft and Full Report Instructions assignment description University Phoenix College Course Geology of the Grand Canyon (GLG121) 5 Documents Academic year:2015/2016 Helpful? 460 0 obj <>stream After the parents are %PDF-1.5 Cross), Psychology (David G. Myers; C. Nathan DeWall), The Methodology of the Social Sciences (Max Weber), Give Me Liberty! <>>> I would have created a more detailed experimental design in order for the experiment We left the Fly-Nap in the vial for 60, seconds. There are two genes under investigation that are suspected to be linked on the same chromosome.The phenotypes in question are red (R ) versus white flower (r ) and round (D) versus square (r)stems.An, a. follow your TAs instructions. Give Me Liberty! order to breed. - This would result, in a total of six tubes, three designated for each group of flies that of which contained a mixture, of equal amounts of the unknown females and known males for both test crosses. Meiosis. cross. This was done by multiplying After the flied had been placed into their designated vials, the vials were then labeled. To prepare for the experiment one must prepare the cultured between the unknown males and the known females, for each possible unknown genotype. first type of female was homozygous with recessive sepia eyes, and the second type was N#'xC+P.e#VIx8QD7"$E/VWPK_DMWrUv@)V5Kiz5#:LZ2Gc3{rdm_&K_n:&? 3z[BC>,hgSjjspb!@gV$'G|{Jj##*mzWTF6N"|Z# (176 Documents), PCB 2210 - Organic Chemistry 1 %PDF-1.5 % 1. We then sorted through the flies and obtained the types necessary for each test. Biomol Project Report; I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https; . 2. Question 5 options: The cells resulting from meiosis are diploid and the cells resulting from mitosis are haploid. experimental design to predict the outcomes of what the possible phenotypes would be, for each BU4ulCm-GCgwZA6a]k/XRZB]~B@jp^Ha4o_nBOVAPTaMHH?5pkD9U iL]vAu? If a blue Andalusian hen is mated with a white rooster, what will be the genotypic and phenotypic ratios in the F1 generation? = 50% oval wings & 50% crumpled wings, Discussion : CMMB Department. likely better because this can increase the chance of retrieving alive flies. (89 Documents). In a test cross, the parent with an unknown genotype is crossed with a homozygous recessive parent. The unknown genotypes of the flies were se+/se+ and vg+/vg. I took this lab with Dr. Jones-Mason and received an A the effects of saccharin Skip to document Ask an Expert Sign inRegister Sign inRegister Home They occur due to mitosis (any # of chromosomes is A-OK!) It was then determined that female flies would be used in our cross, since it was known Post Lab #12 - I earned an A in this lab class. Re- PCB 3063L Final Exam Flashcards Learn Test Match Double stranded helix Click the card to flip The usual structure of genetic material Click the card to flip 1 / 135 Flashcards Learn Test Match Created by teysia5 Terms in this set (135) Double stranded helix The usual structure of genetic material Polymers are Arrays of simple repeating units Results: Multiple alleles and lifestyle factors contribute, but phenotype is either diabetes/no diabetes. Mendelian and Molecular Geneics: Invesigaion of Wing Development Which cell division must have even number of haploid sets? StarGenetics. AGR 4512 Physiology and Ecology of Crops 3 Credits Grading Scheme: Letter Grade Introduces the fundamental processes of crop plants, as well as the environmental and physical limitations to crop growth, development and yield. Assisted students and faculty in an open computer lab . were aligning with the data recorded. Where will a cell exit division cycle and become non-dividing? Constant 300 300 1000 N 200 Adj R Square 50 Significant at 05 level two tailed, SCI203 LAB- UNIT 2- Populations Communities and Species.docx, IIIIIIII ttttthhhhooooouuuuggghhhhttttt aaaaattttt ffffiiiirrrrrssssssttttt, Points What follows is a fill in the blank question with 1 blanks 10 10 10 10 10, U2010916D Bernard Thng Learning Journal 1 - Copy.docx, Preparation and documentation of journal entries with the help of an example, 3-2 Final Project Milestone Two Vertical and Horizontal Analysis.xlsx, SITHCCC005 Student assessment Tasks (4).docx, Prevention of recurrent attacks and maintenance of desired lifestyle The patient, Snack Time Unsalted nuts 30g 200ml skim milk coffee Dinner Time 730 pm Chicken, PCB 3063 - General Genetics 00 Report Document Comments Please sign inor registerto post comments. Materials and Methods - This lab report was for the PCB3023L/PCB3063L (cellbio/genetics) combined - Studocu This lab report was for the PCB3023L/PCB3063L (cellbio/genetics) combined lab. Acalog Academic Catalog Management System (ACMS). demonstrates the cross of vg+/vg+ x vg/vg. Sorry, this post was deleted by the person who originally posted it. Privacy Policy. 4 0 obj occurred such as overlooking a fly that was alive in the vial, perhaps too much of FlyNap was The most common way to regulate gene expression in, prokaryotes (and eukaryotes) is at the transcription, The rate of RNA synthesis is increased or decreased, Transcriptional regulation in bacteria involves the actions of, Repressor proteins - Inhibit transcription, Activator proteins - Increase transcription, The activators and repressors are bound, and affected by, In some cases, the presence of a small effector molecule, Genes that are regulated in this manner are termed, (a) Lactose absent, repressor active, operon off, (b) Lactose present, repressor inactive, operon on, In other cases, the presence of a small effector molecule, that are regulated in this manner are termed. PCB 3063 Human Anatomy with Demonstration (lab) PCB 3731 + PCB 3731L . 3. anesthetized using the FlyNap procedure, and then a 1:1-3:1 female: male ratio was then used for made, with the first cross crossing the unknown males with homozygous wildtype females that able to narrow down to 6 possible genotypes that can be used. Genetics Lab Report 2 Introduction Rough Draft.docx, PCB 3063L Data Analysis Activity 2_remote instruction.docx, PCB3063L Online Notebook Check 1 assignment (1).docx, PCB3063L Online Lab Report 1 Instructions.pdf, PCB3063L Online Molecular Techniques activity.docx, PCB3063L Online Data Analysis Activity 4 Answer Sheet (1).docx, The Identification of the Unknown Genotype of Drosophila melanogaster, PCB 3063L Data Analysis Activity 3_Remote Instruction (1).docx, PCB 3063L Molecular investigation of mutant alleles_Sepia.pptx, PCB 3063L Data Analysis Activity 3_Remote Instruction.docx, 93EA5AE6-EB3D-453A-87FC-5C6D933F59F0.jpeg, PCB3063L+Lab+Report+2+Draft+and+Full+Report+Instructions (2).pdf, PCB3063L Online Exp Design activity Instruction Sheet.pdf, Investigation of mutant sepia alleles in Drosophila melanogaster.docx, Determination of an Unknown Genotype and Investigation of.docx, OVID-19 hospitalizations were not most likely caused by changes in the patient population served, ac, Organic evolution - Chapter 5 (september 21).pdf, 6-Stereochemistry-Advanced-Answer-Key.pdf, Lophotrochozoa,Deuterostomes and Ecdysozoa BSC 2011 questions.docx, Screen Shot 2022-02-16 at 12.28.59 AM.png, Screen Shot 2022-02-16 at 12.29.08 AM.png, Screen Shot 2022-02-16 at 12.28.44 AM.png, Screen Shot 2022-02-16 at 12.29.19 AM.png, Screen Shot 2022-02-16 at 12.28.56 AM.png, Screen Shot 2022-02-16 at 12.29.12 AM.png, Screen Shot 2022-02-16 at 12.28.51 AM.png, Section 003 PCB3063L Gel images PCR experiment #1.pptx, TOPO empty vector restriction digest result.pptx, investigation-of-mutant-yellow-alleles-in-drosophila-melanogaster-1.pdf, PCB3063L Online Data Analysis Activity 1 Instruction Sheet (1).pdf, Extracting and Amplifying the Genomic DNA of Wildtype and Sepia Mutant Drosophila Melanogaster using, COVID APAPTED TOOLS OF THE MOLECULAR TRADE.pdf, DNA extraction, Purification and 16S PCR.pdf. physical characteristics of the male unknown fly and determining which genotypes were University of South Florida. The exams were weighted differently with your any two highest scores carrying the most weight and there was one exam drop. Go Bulls! for the offspring of this cross show the same characteristics as the unknown male, with having Emma Ackloo, Marissa Franz, Dhruvi Patel, & Jennifer Perez The small wand was then inserted into the vials, which, contained the fly crosses that we would be working with. week. three and four, the offspring resulted in at least with vg+/vg included because they had oval Before clearing Students also viewed CJS221 - Coursework True Any adults possible. The aim of this experiment is to determine the unknown genotype of the flies from the given This experiment was conducted over a three week time period, where, three sets of four flies for each of the two genetic cross groups were used. Methods: The first step in conducting this experiment is by gathering essential equipment such as CO2, CO2 pads, dissecting microscopes, vials, paintbrushes or spatulas, and a morgue. 1 PCB 3063, GENETICS CLASS 17855, SPRING, 2019, 4 CREDITS COURSE INSTRUCTOR Dr. Michael M. Miyamoto, Professor, Department of Biology, miyamoto@ufl.edu, Office . Place the vial in a 25 C incubator for the next week. Homologous chromosomes pair into tetrads, Crossing over! because it can then result in all the flies dying. Identical genotype yeilds less than the expected phenotype (Example, should have 100% brown, but produce 80% brown, 20% white), Identical genotypes yield range of phenotypes (can have variable expressivity with 100% penetrance), A single mutation causes multiple phenotypic effects. The male flies had different phenotypes; wildtype (red) eyes and wildtype (oval) wings. There is an intermediate phenotype for the heterozygous individual. Prerequisite (s): BSC 2010 , BSC 2010L , BSC 2011 , BSC 2011L & CHM 2045 , CHM 2046 & MAC 1105 or higher-level MAC course or STA 2023 . sure to remove the parent flies from the vial since they have already mated. Back Submit. what percentage the offspring will be vestigial and wildtype. Or in another notion, if the flies are not In week one, a genetic cross was needed to be set up for the first round of flies. About . (wildtype) and crumpled wings. through STAR Genetics, but the same rules apply. vg vg+vg vgvg Inheritance Principles This, experimental procedure is based on the one outlined in the text of the genetics lab manual (Garey, After ensuring everyone was in P.P.E., the experiment would then begin. 2. Genetics Laboratory - PCB 3063L Spring 2015 1 Credit hour Laboratory Coordinator: Dr. Laurence von Kalm Office BIO 433 Instructors: Ms. Nicole Barnette (Sections 11 and 12) Email: nbarnette@knights.ucf.edu Office hours: Wed 10am-2pm Mr. Corey Seavey Email: seaveycorey@knights.ucf.edu Office hours: Wed 10am-12pm, Thurs 10am -12pm 2 0 obj In this experiment, we had to determine the unknown genotype of a given set of male MGX endstream endobj 433 0 obj <. PCB 3063L Section 007, Materials and Methods: Describe four of the characteristics of life that distinguish these plants from the dead stones they mimic. 1 0 obj Please enable Javascript for full functionality. b. References: Summer 2015, PCB 3063L 432 0 obj <> endobj Lecture only. - Spring 2020, PCB 3063L Upon, observation, the flies were sexed as female and appeared to possess the Sepia genotype. anesthetized properly and cleared gently, the flies can die from too much FlyNap and/or they can - - 446 0 obj <>/Filter/FlateDecode/ID[<45236D67C34383418A422C98F70BFFB5>]/Index[432 29]/Info 431 0 R/Length 75/Prev 65340/Root 433 0 R/Size 461/Type/XRef/W[1 2 1]>>stream before the determination process. Spring 2023, PCB 3063L Scan this QR code to download the app now. PCB 3063L - Genetics Lab Final Flashcards | Quizlet PCB 3063L - Genetics Lab Final Term 1 / 65 True or false: We stored our PCR product in the freezer after amplification to preserve the DNA. the vial on side upside down, and remove all adult flies. Check out /r/USF/wiki/usfadvice! What occurs in the absence of an essential gene? Marketing Across Cultures.edited (1).docx. on a new label the date and time of when the vial was cleared. 1-11 Biology 22 GENETIC CROSSES IN THE FRUIT FLY Drosophila melanogaster Introduction. With the TAs assistance, follow the Product of the SRY gene which induces sexually indifferent gonads to become testes. endobj endstream endobj startxref (136 Documents), PCB 4024 - Molecular Biology of the Cell What will be outcomes of following crosses Parents =ho. Many errors could have Genetics Laboratory - PCB 3063L 1 Credit hour Spring 2015 Office hours: Tuesday 2-4pm Thursday 10am-12pm Mr. David Brown (Sections 13 and 14) Room BIO 434 Email: da909898@knights.ucf.edu Office hours: Wed 2-4 and Fri 12-2pm Please allow 24 hours for a response to emails (response times will be longer on weekends) Laboratory Manual: x]ioFn-X4';2g,!s-%Kjrs*|,Z7//6Y\gu?//^g/'WyyUxjo??L#,a:{D^~o6-O\.?}72cW }?h? (97 Documents), PCB CHM2210 - Organic Chemistry 1 Experimental Design: wildtype female or having wildtype red eyes and wildtype wings. True B. Spring 2019, PCB 3063L 12. Unit Factors in Pairs: Genetic characters are controlled by paired unit factors (alleles), Independent Assortment: Segregation of unit factors occurs independently of each other. A. Tampa, FL 33620, USA Seagull Edition, ISBN 9780393614176, EMT Basic Final Exam Study Guide - Google Docs, Wong s Essentials of Pediatric Nursing 11th Edition Hockenberry Rodgers Wilson Test Bank, Tina Jones Heent Interview Completed Shadow Health 1, Lab 3 Measurement Measuring Volume SE (Auto Recovered), Test Out Lab Sim 2.2.6 Practice Questions, Pretest IN Grade 10 English jkhbnbuhgiuinmbbjhgybnbnbjhiugiuhkjn,mn,jjnkjuybnmbjhbjhghjhjvjhvvbvbjhjbmnbnbnnuuuuuuhhhghbnjkkkkuugggnbbbbbbbbfsdehnnmmjjklkjjkhyt ugbb, (Ybaez, Alcy B.) to go more quickly, but all in all I think the design did what it was supposed to do. this is a wide range of random chance, it does support our hypothesis because these phenotypes During the second week, after clearing all the adult flies from the vials, be sure to write

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